豬腫瘤壞死因子α(TNF-α) ELISA試劑盒,Porcine TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit 生命科學產品與技術服務-必威betway官方网站登录工程(上海)股份有限公司 - betway888,必威betway官方网站登录

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Order NO. D741029

豬腫瘤壞死因子α(TNF-α) ELISA試劑盒

英文名	Porcine TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
別名	DIF,TNF,TNF-alpha,TNFA,TNFSF2,TNFa
相關類別	ELISA	儲存	按標簽提示分開儲存
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編號	規格	庫存	目錄價（￥）	您的價格（￥）	數量
D741029-0048	48 TESTS	預計3日內發貨	待詢價	待詢價	－ +
D741029-0096	96 TESTS	預計5日內發貨	待詢價	待詢價	－ +

產品描述

應用

用於豬血清、血漿或其他相關生物液體中腫瘤壞死因子α的測定
For the determination of Porcine TNF-α(Tumor Necrosis Factor Alpha) concentrations in serum, plasma and other related biological fluids.

屬性

工作原理	本試劑盒采用的是雙抗體夾心法酶聯免疫吸附檢測技術（ELISA）。測定樣品中豬腫瘤壞死因子α水平。向預先包被了抗豬腫瘤壞死因子α抗體的酶標孔中，加入標準品或樣本，溫育後，加入生物素標記的抗腫瘤壞死因子α抗體。再與HRP標記的鏈黴親和素結合，形成免疫複合物，再經過溫育和洗滌，去除未結合的酶，然後加入顯色底物TMB，產生藍色，並在酸的作用下轉化成最終的黃色。最後，在450 nm處測定反應孔樣品吸光度（OD）值，樣本中的豬腫瘤壞死因子α濃度與OD值成正比，通過繪製標準曲線計算出樣本中豬腫瘤壞死因子α的濃度。
Purpose	This assay employs the Sandwich enzyme immunoassay technique. Determine the level of Porcine TNF-α(Tumor Necrosis Factor Alpha) in the sample. Add standard or sample to the microplate that have been pre-coated with Anti-Porcine TNF-α(Tumor Necrosis Factor Alpha) antibody. After incubation, add Biotin-conjugated anti-Porcine TNF-α(Tumor Necrosis Factor Alpha) antibody. It is then combined with HRP-conjugated streptavidin to form an immune complex, then incubated and washed to remove unbound enzyme, and then added to the chromogenic substrate TMB to produce a blue color, and converted to the final yellow under the action of acid. Finally, the absorbance (OD) value was measured at 450 nm. The concentration of Porcine TNF-α(Tumor Necrosis Factor Alpha) in the sample was proportional to the OD value. The concentration of Porcine TNF-α(Tumor Necrosis Factor Alpha) in the sample can be calculated by drawing a standard curve.
靈敏度	18.75 pg/mL
Sensitivity	18.75 pg/mL
檢測範圍	31.25-2000 pg/mL
Examination range	31.25-2000 pg/mL
特異性	可檢測樣本中的豬腫瘤壞死因子α，且與其類似物無明顯交叉反應。
Specificity	This kit recognizes Porcine TNF-α(Tumor Necrosis Factor Alpha) in samples. No significant cross-reactivity or interference between Porcine TNF-α(Tumor Necrosis Factor Alpha) and analogues was observed.
重複性	三種不同濃度的樣本在同一塊板子上分別檢測20次，以及在不同的板子上分別檢測20次，板內和板間變異係數均小於10%。
Repeatability	Three different concentrations of samples were detected 20 times on the same board and 20 times on different boards. Within the plate or between the plates, the coefficient of variation is <10%.
回收率	分別往稀釋不同倍數的5個樣本中加入已知濃度的目標蛋白，做回收實驗，得出回收率範圍和平均回收率。
Recovery	A known concentration of the target protein was added to 5 samples which were diluted to different multiples, and a recovery experiment was performed to obtain a recovery range and an average recovery rate.

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