概述

The Protein A magnetic beads, conjugated with protein A, are used for IP or purification of many species of IgG (Antibody) , especially for Human IgG, IgG1, IgG2, IgG4, Rabbit IgG, Mouse IgG2a, Cat IgG, Goat IgG2, Sheep IgG2, Cow IgG2, Pig IgG, and so on. For purification, the antibody is added to a tube containing Protein A purified beads. The tube is then placed on a Magnetic separation device, where the beads migrate to the side wall of the tube facing the Magnetic separation device and allow for easy removal of the supernatant. The bound antibodies are dissociated from the beads using an Elution Buffer.
蛋白A磁珠是磁珠與蛋白A偶聯，可用於免疫沉澱或純化多種 IgG（抗體），尤其是人 IgG、IgG1、IgG2、IgG4、兔 IgG、小鼠 IgG2a、貓 IgG、山羊 IgG2、綿羊 IgG2、 牛 IgG2、豬 IgG 等。 為了純化，將抗體加入含有蛋白A磁珠的離心管中。 然後將管放置在磁分離裝置上，磁珠遷移到管內麵向磁分離裝置的側壁，使用洗脫緩衝液將結合的抗體與磁珠分離。

應用

IP; Purification

屬性