pET-32b（+）大腸杆菌表達載體,pET-32b（+）DNA 生命科學產品與技術服務-必威betway官方网站登录工程(上海)股份有限公司

Order NO. B540187

pET-32b（+）大腸杆菌表達載體

英文名	pET-32b（+）DNA
相關類別	載體與菌株	儲存	冷凍（-20℃）
在線谘詢

編號	規格	庫存	目錄價（￥）	您的價格（￥）	數量
B540187-0001	10 UG	現貨	831	831	－ +

產品描述

概述

The pET-32a-c series is designed for cloning and high-level expression of peptide sequences fused with the 109aa Trx?Tag? thioredoxin protein (1). Cloning sites are available for producing fusion proteins also containing cleavable His?Tag? and S?Tag? sequences for detection and purification. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer.

屬性

質粒類型	大腸杆菌表達載體
表達水平	高
克隆方法	多克隆位點，限製性內切酶
載體大小	5899 bp
5'測序引物及序列	T7：5'-TAATACGACTCACTATAGGG-3'
3'測序引物及序列	T7t：5'-GCTAGTTATTGCTCAGCGG-3'
載體標簽	N-T7，C-His
載體抗性	氨苄青黴素（Ampicillin）
備注	Production of soluble, active target proteins; N-term thrombin cleavage site; Nterm enterokinase cleavage site; a,b,c vary by MCS

相關文檔

質量控製相關文件

質量報告 (COA)