Order NO. D191001
小鼠抗6X His單克隆抗體
英文名 | Anti-6×His Tag mouse monoclonal antibody | ||
別名 | 6 His epitope tag | ||
相關類別 | 標簽抗體 | 儲存 | 冷凍(-20℃) 避光 |
宿主 | Mouse | 反應種屬 | Fusion proteins containing the His Tag |
標記物 | Unconjugate | 克隆類型 | mouse monoclonal |
抗原 | 6×His Tag | ||
編 號 | 包裝 | 庫存 | 目錄價(¥) | 您的價格(¥) | 數 量 |
D191001-0025 | 25 ul | 現貨 |
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D191001-0100 | 100 ul | 現貨 |
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D191001-0200 | 200 ul | 現貨 |
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技術規格
Background: | The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification. It can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting. The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells. |
Applications: | WB, IF, IP |
Name of antibody: | 6×His Tag |
Immunogen: | Synthetic peptide conjugated with KLH |
Full name: | HHHHHH Tag |
Synonyms: | 6 His epitope tag |
WB Predicted band size: | 50 kDa |
WB Positive control: | Fusion proteins containing the His Tag |
WB Recommended dilution: | 1000-5000 |
IF Positive control: | 3T3 cells |
IF Recommended dilution: | 100-500 |
IP Recommended dilution: | 100-200 |