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Order NO. C610387
重組大鼠PDGF-AA
英文名 Recombinant Rat Platelet-derived Growth Factor-AA
別名 PDGF-AA, Rat
相關類別 其他重組蛋白 儲存 冷凍(-20℃)
分子量 Approximately 25.3 kDa, a disulfide-linked homodimeric protein containing two 111 amino acid residues polypeptide (A chain).
編 號 包裝 庫存 目錄價(¥) 您的價格(¥) 數 量
C610387-0002 2 UG 預計20日內發貨
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C610387-0010 10 UG 現貨
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產品描述

概述

Platelet-derived growth factor (PDGF) presenting in serum but absent from plasma was first discovered in animal study by Lynch and co-workers in the late 1980s. It is a disulfide-linked dimer consisting of two peptides-chain A and chain B. PDGF has three subforms: PDGF-AA, PDGF-BB, PDGF-AB. It is involved in a number of biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The function of PDGF is mediated by two receptors (PDGFR-α and PDGFR-β).

使用說明

This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

技術規格

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Purity > 98 % by SDS-PAGE and HPLC analyses.
Source Escherichia coli.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0 × 105 IU/mg.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 5 % Trehalose.
Endotoxin Less than 0.1 EU/μg of rRtPDGF-AA as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.
AA Sequence MSIEEAIPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETDV R
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