英文名 | SMCC | ||
別名 | SMCC | ||
相關類別 | 蛋白異源雙功能交鏈試劑 | 儲存 | 冷藏(2-8℃) |
分子量 | 334.3 | 無 | 4-(N-馬來酰亞胺基甲基)環己烷-1-羧酸琥珀酰亞胺酯 |
編 號 | 包裝 | 庫存 | 目錄價(¥) | 您的價格(¥) | 數 量 |
C100317-0050 | 50 MG | 現貨 |
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概述
SMCC and its water-soluble analog Sulfo-SMCC are heterobifunctionalcrosslinkers that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7~9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5~7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group but will slowly hydrolyze and loses its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2~7.5, with the NHS-ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryltargeted) reaction.
應用
SMCC is not directly water-soluble and must be dissolved in an organic solvent such as dimethylsulfoxide (DMSO) or dimethylformamide (DMF); subsequent dilution into aqueous reaction buffer is generally.possible, and most protein reactants will remain soluble if the final concentration of organic solvent is less than 10%.
組份
50 mg/bottle
技術規格
Molecular Weight | 334.32 |
Spacer arm | 8.3 |
Purity | >98% on TLC |
Net Mass Added | 219.09 |