英文名 | Sulfo-MBS | ||
別名 | 3-Maleimidobenzoyl-N-hydroxysulfosuccinimide ester | ||
相關類別 | 蛋白異源雙功能交鏈試劑 | 儲存 | 冷凍(-20℃) 避光+幹燥 |
分子量 | 416.2 | 無 | 3-馬來酰亞胺基苯甲酸琥珀酰亞胺酯(水溶性) |
編 號 | 包裝 | 庫存 | 目錄價(¥) | 您的價格(¥) | 數 量 |
C100314-0050 | 50 MG | 現貨 |
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概述
MBS and its water-soluble analog Sulfo-MBS are heterobifunctional cross-linkers that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7-9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. In aqueous solutions, hydrolytic degradation of the NHS ester is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group but will slowly hydrolyze and also lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugation experiments involving these cross-linkers are usually performed at pH 7.2-7.5, with the NHS-ester (amine-targeted) reaction being accomplished before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.
應用
Sulfo-MBS is soluble in water and many other aqueous buffers to approximately 10 mM, although solubility decreases with increasing salt concentration
組份
50 mg/bottle
技術規格
Molecular Weight | 416.3 |
Spacer arm | 7.3 |
Purity | >95% on TLC |